Ohio Advanced EPR Laboratory at Miami University

A resource serving the pulsed and CW EPR needs of the greater Ohio area.

Structural Investigation of the Transmembrane Domain of KCNE1 in Proteoliposomes Sahu, Indra Dev; Kroncke, Brett M.; Zhang, Rongfu; Dunagan, Megan M.; Smith, Hubbell J.; Craig, Andrew; McCarrick, Robert M.; Sanders, Charles R.; Lorigan, Gary A. Biochemistry (2014)

Dilution of dipolar interactions in a spin-labeled, multimeric metalloenzyme for DEER studies Aitha, Mahesh; Richmond, Timothy K.; Hu, Zhenxin; Hetrick, Alyssa; Reese, Raquel; Gunther, Althea; McCarrick, Robert; Bennett, Brian; Crowder, Michael W. Journal of Inorganic Biochemistry (2014), 136, 40-46.

Microbial reduction and precipitation of vanadium by mesophilic and thermophilic methanogens Zhang, Jing; Dong, Hailiang; Zhao, Linduo; McCarrick, Robert; Agrawal, Abinash Chemical Geology (2014), 370, 29-39.

Halide-Promoted Dioxygenolysis of a Carbon-Carbon Bond by a Copper(II) Diketonate Complex Allpress, Caleb J.; Milaczewska, Anna; Borowski, Tomasz; Bennett, Jami R.; Tierney, David L.; Arif, Atta M.; Berreau, Lisa M. Journal of the American Chemical Society (2014), 136(22), 7821-7824

Spectroscopic and mechanistic studies of heterodimetallic forms of metallo-β-lactamase NDM-1 Yang, Hao; Aitha, Mahesh; Marts, Amy R.; Hetrick, Alyssa; Bennett, Brian; Crowder, Michael W.; Tierney, David L. Journal of the American Chemical Society (2014), 136(20), 7273-7285. 

DEER EPR Measurements for Membrane Protein Structures via Bifunctional Spin Labels and Lipodisq Nanoparticles Sahu, Indra D.; McCarrick, Robert M.; Troxel, Kaylee R.; Zhang, Rongfu; Smith, Hubbell J.; Dunagan, Megan M.; Swartz, Max S.; Rajan, Prashant V.; Kroncke, Brett M.; Sanders, Charles R.; Lorigan, Gary A. Biochemistry (2013), 52(38), 6627-6632.

Use of Electron Paramagnetic Resonance To Solve Biochemical Problems Sahu, Indra D.; McCarrick, Robert M.; Lorigan, Gary A. Biochemistry (2013), 52(35), 5967-5984.

Measurement of rate constants for homodimer subunit exchange using double electron-electron resonance and paramagnetic relaxation enhancements Yang, Yunhuang; Ramelot, Theresa A.; Ni, Shuisong; McCarrick, Robert M.; Kennedy, Michael A. Journal of Biomolecular NMR (2013), 55(1), 47-58.

Determining α-Helical and β-Sheet Secondary Structures via Pulsed Electron Spin Resonance Spectroscopy Zhou, Andy; Abu-Baker, Shadi; Sahu, Indra D.; Liu, Lishan; McCarrick, Robert M.; Dabney-Smith, Carole; Lorigan, Gary A. Biochemistry (2012), 51(38), 7417-7419. 

Enhancement of Electron Spin Echo Envelope Modulation Spectroscopic Methods to Investigate the Secondary Structure of Membrane Proteins Liu, Lishan; Sahu, Indra D.; Mayo, Daniel J.; McCarrick, Robert M.; Troxel, Kaylee; Zhou, Andy; Shockley, Erin; Lorigan, Gary A. Journal of Physical Chemistry B (2012), 116(36), 11041-11045.

Photoinitiated Dioxygenase-Type Reactivity of Open-Shell 3d Divalent Metal Flavonolato Complexes Grubel, Katarzyna; Marts, Amy R.; Greer, Samuel M.; Tierney, David L.; Allpress, Caleb J.; Anderson, Stacey N.; Laughlin, Brynna J.; Smith, Rhett C.; Arif, Atta M.; Berreau, Lisa M. European Journal of Inorganic Chemistry (2012), 2012(29), 4750-4757.

Stress response and virulence functions of the Acinetobacter baumannii NfuA Fe-S scaffold protein Zimbler, Daniel L.; Park, Thomas M.; Arivett, Brock A.; Penwell, William F.; Greer, Samuel M.; Woodruff, Tessa M.; Tierney, David L.; Actis, Luis A. Journal of Bacteriology (2012), 194(11), 2884-2893.

The influence of spin-labeled fluorene compounds on the assembly and toxicity of the Aβ peptide Petrlova, Jitka; Kalai, Tamas; Maezawa, Izumi; Altman, Robin; Harishchandra, Ghimire; Hong, Hyun-Seok; Bricarello, Daniel A.; Parikh, Atul N.; Lorigan, Gary A.; Jin, Lee-Way;  Hideg, Kalman; Voss, John C. PLoS One (2012), 7(4), e35443.

Differential Binding between PatS C-Terminal Peptide Fragments and HetR from Anabaena sp. PCC 7120 Feldmann, Erik A.; Ni, Shuisong; Sahu, Indra D.; Mishler, Clay H.; Levengood, Jeffrey D.; Kushnir, Yegor; McCarrick, Robert M.; Lorigan, Gary A.; Tolbert, Blanton S.; Callahan, Sean M.; Kennedy, Michael A. Biochemistry (2012), 51(12), 2436-2442.

Distance Measurements on a Dual-Labeled TOAC AChR M2δ Peptide in Mechanically Aligned DMPC Bilayers via Dipolar Broadening CW-EPR Spectroscopy Ghimire, Harishchandra; Hustedt, Eric J.; Sahu, Indra D.; Inbaraj, Johnson J.; McCarrick, Robert; Mayo, Daniel J.; Benedikt, Monica R.; Lee, Ryan T.; Grosser, Stuart M.; Lorigan, Gary A. Journal of Physical Chemistry B (2012), 116(12), 3866-3873.

Crowded bis ligand complexes of TtzPh,Me with first row transition metals rearrange due to ligand field effects: structural and electronic characterization (TtzPh,Me = tris(3-phenyl-5-methyl-1,2,4-triazolyl)borate) Oseback, Shannon N.; Shim, Sarah W.; Kumar, Mukesh; Greer, Samuel M.; Gardner, Sean R.; Lemar, Keisha M.; DeGregory, Paul R.; Papish, Elizabeth T.; Tierney, David L.; Zeller, Matthias;  Yap, Glenn P. A. Dalton Transactions (2012), 41(9), 2774-2787.

Time-resolved EPR immersion depth studies of a transmembrane peptide incorporated into bicelles Nusair, Nisreen A.; Mayo, Daniel J.; Dorozenski, Tia D.; Cardon, Thomas B.; Inbaraj, Johnson J.; Karp, Ethan S.; Newstadt, Justin P.; Grosser, Stuart M.; Lorigan, Gary A. Biochimica et Biophysica Acta, Biomembranes (2012), 1818(3), 821-828.

Probing the helical tilt and dynamic properties of membrane-bound phospholamban in magnetically aligned bicelles using electron paramagnetic resonance spectroscopy Ghimire, Harishchandra; Abu-Baker, Shadi; Sahu, Indra D.; Zhou, Andy; Mayo, Daniel J.; Lee, Ryan T.; Lorigan, Gary A. Biochimica et Biophysica Acta, Biomembranes (2012), 1818(3), 645-650.

Reconstitution of KCNE1 into Lipid Bilayers: Comparing the Structural, Dynamic, and Activity Differences in Micelle and Vesicle Environments Coey, Aaron T.; Sahu, Indra D.; Gunasekera, Thusitha S.; Troxel, Kaylee R.; Hawn, Jaclyn M.; Swartz, Max S.; Wickenheiser, Marilyn R.; Reid, Ro-jay; Welch, Richard C.; Vanoye, Carlos G.;  Kang, Congbao; Sanders, Charles R.; Lorigan, Gary A. Biochemistry (2011), 50(50), 10851-10859.

Structural and kinetic studies on metallo-β-lactamase IMP-1 Griffin, Dionne H.; Richmond, Timothy K.; Sanchez, Carlo; Moller, Abraham Jon; Breece, Robert M.; Tierney, David L.; Bennett, Brian; Crowder, Michael W. Biochemistry (2011), 50(42), 9125-9134.

Evidence for Direct Binding between HetR from Anabaena sp. PCC 7120 and PatS-5 Feldmann, Erik A.; Ni, Shuisong; Sahu, Indra D.; Mishler, Clay H.; Risser, Douglas D.; Murakami, Jodi L.; Tom, Sasa K.; McCarrick, Robert M.; Lorigan, Gary A.; Tolbert, Blanton S.;  Callahan, Sean M.; Kennedy, Michael A. Biochemistry (2011), 50(43), 9212-9224.

Dual Mode EPR Studies of a Kramers ion: High-Spin Co(II) in 4-, 5- and 6-Coordination Marts, Amy R.; Greer, Samuel M.; Whitehead, Daniel R.; Woodruff, Tessa M.; Breece, Robert M.; Shim, Sarah W.; Oseback, Shannon N.; Papish, Elizabeth T.; Jacobsen, Faith E.; Cohen, Seth M.; Tierney, David L. Applied Magnetic Resonance (2011), 40(4), 501-511.

Probing the structure of membrane proteins with electron spin echo envelope modulation spectroscopy Mayo, Daniel; Zhou, Andy; Sahu, Indra; McCarrick, Robert; Walton, Parker; Ring, Adam; Troxel, Kaylee; Coey, Aaron; Hawn, Jaclyn; Emwas, Abdul-Hamid;  Lorigan, Gary A. Protein Science (2011), 20(7), 1100-1104.

A differential association of apolipoprotein E isoforms with the amyloid-β oligomer in solution Petrlova, Jitka; Hong, Hyun-Seok; Bricarello, Daniel A.; Harishchandra, Ghimire; Lorigan, Gary A.; Jin, Lee-Way; Voss, John C. Proteins: Structure, Function, and Bioinformatics (2011), 79(2), 402-416.

What Is the True Color of Fresh Meat? A Biophysical Undergraduate Laboratory Experiment Investigating the Effects of Ligand Binding on Myoglobin Using Optical, EPR, and NMR Spectroscopy Linenberger, Kimberly; Bretz, Stacey Lowery; Crowder, Michael W.; McCarrick, Robert; Lorigan, Gary A.; Tierney, David L. Journal of Chemical Education (2011), 88(2), 223-225.

Converting GLX2-1 into an Active Glyoxalase II Limphong, Pattraranee; Adams, Nicole E.; Rouhier, Matthew F.; McKinney, Ross M.; Naylor, Melissa; Bennett, Brian; Makaroff, Christopher A.; Crowder, Michael W. Biochemistry (2010), 49(37), 8228-8236.

Combining NMR and EPR Methods for Homodimer Protein Structure Determination Yang, Yunhuang; Ramelot, Theresa A.; McCarrick, Robert M.; Ni, Shuisong; Feldmann, Erik A.; Cort, John R.; Wang, Huang; Ciccosanti, Colleen; Jiang, Mei; Janjua, Haleema; Acton, Thomas B.; Xiao, Rong; Everett, John K.; Montelione, Gaetano T.; Kennedy, Michael A. Journal of the American Chemical Society (2010), 132(34), 11910-11913.

The metal ion requirements of Arabidopsis thaliana Glx2-2 for catalytic activity Limphong, Pattraranee; McKinney, Ross M.; Adams, Nicole E.; Makaroff, Christopher A.; Bennett, Brian; Crowder, Michael W. JBIC, Journal of Biological Inorganic Chemistry (2010), 15(2), 249-258.

Anisotropic Fermi Couplings Due to Large Unquenched Orbital Angular Momentum: Q-Band 1H, 14N, and 11B ENDOR of Bis(trispyrazolylborate) Cobalt(II) Myers, William K.; Scholes, Charles P.; Tierney, David L. Journal of the American Chemical Society (2009), 131(30), 10421-10429.

Differential Binding of Co(II) and Zn(II) to Metallo-β-Lactamase Bla2 from Bacillus anthracis Hawk, Megan J.; Breece, Robert M.; Hajdin, Christine E.; Bender, Katherine M.; Hu, Zhenxin; Costello, Alison L.; Bennett, Brian; Tierney, David L.; Crowder, Michael W. Journal of the American Chemical Society (2009), 131(30), 10753-10762.

A comparative study of the effect of cholesterol on bicelle model membranes using X-band and Q-band EPR spectroscopy Ghimire, Harishchandra; Inbaraj, Johnson J.; Lorigan, Gary A. Chemistry and Physics of Lipids (2009), 160(2), 98-104.

Significantly Improved Sensitivity of Q-Band PELDOR/DEER Experiments Relative to X-Band Is Observed in Measuring the Intercoil Distance of a Leucine Zipper Motif Peptide (GCN4-LZ) Ghimire, Harishchandra; McCarrick, Robert M.; Budil, David E.; Lorigan, Gary A. Biochemistry (2009), 48(25), 5782-5784.

Miami University

Department of Chemistry and Biochemistry

Ohio Advanced EPR Laboratory
701 East High Street
101 Hughes Laboratories
Oxford, OH 45056

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